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Mass Spectrometry Reveals the Assembly Pathway of Encapsulated Ferritins and Highlights a Dynamic Ferroxidase Interface

preprint
submitted on 25.09.2019 and posted on 27.09.2019 by Jennifer Ross, Thomas Lambert, Cecilia Piergentili, Didi He, Kevin J. Waldron, C. Logan Mackay, Jon Marles-Wright, David Clarke
Encapsulated ferritins (EncFtn) are a recently characterised member of the ferritin superfamily. EncFtn proteins are sequestered within encapsulin nanocompartments and form a unique biological iron storage system. Here, we use native mass spectrometry and hydrogen-deuterium exchange mass spectrometry to elucidate the metal-mediated assembly pathway of EncFtn.

Funding

BB/N005570/1

BB/R013993/1

History

Email Address of Submitting Author

dave.clarke@ed.ac.uk

Institution

University of Edinburgh

Country

United Kingdom

ORCID For Submitting Author

0000-0002-3741-2952

Declaration of Conflict of Interest

No conflict of Interest.

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