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Magnetically Responsive Horseradish peroxidase@ZIF-8 for Biocatalysts

preprint
submitted on 02.12.2019 and posted on 18.12.2019 by Raffaele Ricco, Heinz Amenitsch, paolo falcaro
Here, we studied a catalytically active and magnetically responsive porous bio-composite obtained from the synthesis of ZIF-8 in presence of iron oxide magnetic nanoparticles and horseradish peroxidase (HRP) enzyme as guest species. Using a one-pot approach in water the precursors of ZIF-8 (zinc acetate and 2-methylimidazole) spontaneously self-assembles around the guest species. We characterized the composite by means of XRD, SEM, FTIR, AFM, and CLSM. SAXS investigation of the kinetics of crystallization showed how the presence of the guest species can act as nucleation seeds. Moreover, we found that the bio-catalytic activity of the HRP/MNP@ZIF-8 biocomposite is 5 times higher than the analoguous composite without MNPs.

Funding

MNEMONIC 748649

POPCRYSTAL 771834

FETOPEN-1-2016-2017 801464

History

Email Address of Submitting Author

raffaele.ricco@tugraz.at

Institution

Graz University of Technology

Country

Austria

ORCID For Submitting Author

0000-0002-0296-9398

Declaration of Conflict of Interest

There are no conflicts of interest

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