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Local, Single-Molecule Oxidative Cleaving of DNA Origami by C60 on an AFM Tip
preprintsubmitted on 02.02.2021, 08:45 and posted on 03.02.2021, 09:15 by Ankita Ray, Cristiana Passiu, S.N Ramakrishna, Antonella Rossi, Akinori Kuzuya, Nicholas D. Spencer, Yoko Yamakoshi
Spatially controlled single-molecule oxidation of DNA was performed by photocatalytic generation of singlet oxygen on chemically functionalized AFM tips. A waffle-type DNA origami deposited on a mica surface is site-specifically destroyed by generation of reactive oxygen species at the AFM tip, which was modified with C60-tripod photocatalyst. Upon AFM scanning under photoirradiation, DNA morphology changes, corresponding to oxidative damage were clearly observed at the single-molecule level. The DNA cleavage occurred with strict dependence on photoirradiation and the presence of C60 on the AFM tip.