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Local, Single-Molecule Oxidative Cleaving of DNA Origami by C60 on an AFM Tip

preprint
submitted on 02.02.2021, 08:45 and posted on 03.02.2021, 09:15 by Ankita Ray, Cristiana Passiu, S.N Ramakrishna, Antonella Rossi, Akinori Kuzuya, Nicholas D. Spencer, Yoko Yamakoshi
Spatially controlled single-molecule oxidation of DNA was performed by photocatalytic generation of singlet oxygen on chemically functionalized AFM tips. A waffle-type DNA origami deposited on a mica surface is site-specifically destroyed by generation of reactive oxygen species at the AFM tip, which was modified with C60-tripod photocatalyst. Upon AFM scanning under photoirradiation, DNA morphology changes, corresponding to oxidative damage were clearly observed at the single-molecule level. The DNA cleavage occurred with strict dependence on photoirradiation and the presence of C60 on the AFM tip.

Funding

SNF project grant 200021_156097 (Y.Y.)

ETH grant ETH-45 19-1 (Y.Y.)

History

Email Address of Submitting Author

yamakoshi@org.chem.ethz.ch

Institution

ETH Zürich

Country

Switzerland

ORCID For Submitting Author

0000-0001-8466-0118

Declaration of Conflict of Interest

Authors declare no competing interests.

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