Local, Single-Molecule Oxidative Cleaving of DNA Origami by C60 on an AFM Tip

03 February 2021, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Spatially controlled single-molecule oxidation of DNA was performed by photocatalytic generation of singlet oxygen on chemically functionalized AFM tips. A waffle-type DNA origami deposited on a mica surface is site-specifically destroyed by generation of reactive oxygen species at the AFM tip, which was modified with C60-tripod photocatalyst. Upon AFM scanning under photoirradiation, DNA morphology changes, corresponding to oxidative damage were clearly observed at the single-molecule level. The DNA cleavage occurred with strict dependence on photoirradiation and the presence of C60 on the AFM tip.

Keywords

DNA origami
Fullerene C60
nanofabrication
Reactive oxygen species
Singlet oxygen
Atomic force microscopy
PeakForce tapping

Supplementary materials

Title
Description
Actions
Title
supporting new
Description
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.