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Liposomal ChitosanNAcetyl Cysteine Nanoparticle for In-Vitro & In-Vivo Near Infrared Fluorescent (NIR) Imaging.pdf (677.21 kB)
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Liposomal Chitosan-N-Acetyl Cysteine Nanoparticle for In-Vitro/In-Vivo Near Infrared Fluorescent (NIR) Imaging

preprint
submitted on 14.10.2019 and posted on 16.10.2019 by Piyush Kumar, Timothy Van Treuren, Amalendu Ranjan, Jamboor K Vishwanatha

In this work, we studied the effect of chitosan conjugated N-acetyl cysteine (CHT-NAC) coating on liposomal (DMPC:14 lysoPG: DSPE-2000-NH2) nanoparticles as a vehicle to cross the blood-brain barrier. The size of lipo-NP and Lipo-CHT-NAC NP were sub 50 nm with negative surface charge consistent with its use in an intravenous application. In vitro near infrared (NIR) imaging showed good cellular uptake in two triple-negative breast cancer cell lines (MDA-MB-231 and brain metastatic MDA-MB-831). Live (4-120 h) and ex-vivo near-infrared imaging at 24 h in nude mice showed the extended circulation of CHT-NAC Lipo-NP. These results demonstrated that Lipo-CHT-NAC NP could be used for metastatic brain tumor imaging.

Funding

This work was supported by grant awards from National Institutes of Health number R21CA194295 and RO1CA220273 (JKV).

History

Email Address of Submitting Author

krpiyush247@gmail.com

Institution

University of North Texas Health Science Centre

Country

USA

ORCID For Submitting Author

0000-0002-4559-7264

Declaration of Conflict of Interest

No Conflict of Interest

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