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Ion-Mobility Mass Spectrometry of Glycans and Glycopeptides Using Both Intact and Glycan Sequencing Approaches In Positive Ion Mode Facilitates Glycan Isomer and Glycan Motif Identification

preprint
submitted on 17.04.2020 and posted on 20.04.2020 by Edward Pallister, Matthew Choo, ian walsh, jien nee tai, Shi-Jie Tay, Yuansheng Yang, Say-Kong Ng, Pauline Rudd, Sabine Flitsch, Terry Nguyen-Khuong
High resolution glycan analysis has become an important part of biopharmaceutical API production and quality control. Liquid chromatography (LC) is now a well-established technique in this field but the resolution of similar isomeric glycan structures is still a challenge. Here we show that the addition of ion mobility spectroscopy (IMS) in a hyphenated LC-IMS-MS setting allows for the high resolution of N-glycan isomers during positive ion analysis. We have identified unique features in the IM chromatograms to help differentiate a range of isomeric N-glycans for both RFMS labelled glycans and glycopeptides.

Funding

GlycoSing

ARAP

University of Manchester (United Kingdom)

Agency for Science Technology and Research (Singapore)

History

Email Address of Submitting Author

edward.pallister@gmail.com

Institution

University of Manchester

Country

United Kingdom

ORCID For Submitting Author

0000-0002-9681-7327

Declaration of Conflict of Interest

No conflicts of interest

Version Notes

This is an original version of the manuscript

Exports