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Ion-Mobility Mass Spectrometry of Glycans and Glycopeptides Using Both Intact and Glycan Sequencing Approaches In Positive Ion Mode Facilitates Glycan Isomer and Glycan Motif Identification
preprintsubmitted on 17.04.2020, 06:14 and posted on 20.04.2020, 10:50 by Edward Pallister, Matthew Choo, ian walsh, jien nee tai, Shi-Jie Tay, Yuansheng Yang, Say-Kong Ng, Pauline Rudd, Sabine Flitsch, Terry Nguyen-Khuong
High resolution glycan analysis has become an important part of biopharmaceutical API production and quality control. Liquid chromatography (LC) is now a well-established technique in this field but the resolution of similar isomeric glycan structures is still a challenge. Here we show that the addition of ion mobility spectroscopy (IMS) in a hyphenated LC-IMS-MS setting allows for the high resolution of N-glycan isomers during positive ion analysis. We have identified unique features in the IM chromatograms to help differentiate a range of isomeric N-glycans for both RFMS labelled glycans and glycopeptides.