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In Vitro Reconstitution and Kinetic Characterization of the Essential Condensation Enzymes Involved in Prodiginine and Tambjamine Biosynthesis

preprint
submitted on 16.04.2019 and posted on 19.04.2019 by Katherine J. Picott, Julie A. Deichert, Ella M. deKemp, Avena Ross
We have reconstituted the in vitro activity of the purified condensation enzymes PigC and TamQ, from prodiginine and tambjamine pathways respectively. Upon analysis and comparison of their kinetic profiles, a ping-pong reaction sequence with substrate inhibition was identified for each condensation enzyme.

History

Email Address of Submitting Author

avena.ross@queensu.ca

Institution

Queen's University

Country

Canada

ORCID For Submitting Author

0000-0002-9845-729X

Declaration of Conflict of Interest

No conflict of interest

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