In Vitro Reconstitution and Kinetic Characterization of the Essential Condensation Enzymes Involved in Prodiginine and Tambjamine Biosynthesis

We have reconstituted the in vitro activity of the purified condensation enzymes PigC and TamQ, from prodiginine and tambjamine pathways respectively. Upon analysis and comparison of their kinetic profiles, a ping-pong reaction sequence with substrate inhibition was identified for each condensation enzyme.