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In-Plate Metabolomics of Single Zebrafish Embryos

preprint
submitted on 25.10.2019 and posted on 29.10.2019 by Anton Ribbenstedt, Malte Posselt, Carl Brunius, Jonathan P. Benskin
Single embryos were exposed to the beta blocker propranolol for 120 hpf in 96 well plates, during which apical endpoints were determined through microscopy. The water was then removed and analyzed for its exposure concentration and the embryos were frozen on dry ice. The embryos were then extracted within plates and injected directly from the plates using three different LC-MS methods: Flow-injection lipidomics, C18-Orbitrap and HILIC-Orbitrap. Reproducibility was evaluated using non-exposed embryos from four different 96 well plates incubated a year later than the first exposure plate.

History

Email Address of Submitting Author

anton.ribbenstedt@aces.su.se

Institution

ACES-O

Country

Sweden

ORCID For Submitting Author

0000-0002-9985-5644

Declaration of Conflict of Interest

The authors declare no conflicts of interest

Version Notes

Right before submission

Exports