In-Plate Metabolomics of Single Zebrafish Embryos

29 October 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Single embryos were exposed to the beta blocker propranolol for 120 hpf in 96 well plates, during which apical endpoints were determined through microscopy. The water was then removed and analyzed for its exposure concentration and the embryos were frozen on dry ice. The embryos were then extracted within plates and injected directly from the plates using three different LC-MS methods: Flow-injection lipidomics, C18-Orbitrap and HILIC-Orbitrap. Reproducibility was evaluated using non-exposed embryos from four different 96 well plates incubated a year later than the first exposure plate.

Keywords

Non-target
REACH
OECD 236
Orbitrap
Single Embryo
3R
Metabolomics
MoA
Mode of Action
Mechanism of Action
Zebrafish Embryo
Propranolol
Danio Rerio

Supplementary materials

Title
Description
Actions
Title
Supporting Information
Description
Actions
Title
Supporting Tables
Description
Actions
Title
Table S6
Description
Actions
Title
Data S1
Description
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.