ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
CC-origami paper - chemRxiv.pdf (8.73 MB)

Hierarchical Assembly of DNA Origami Nanostructures Using Coiled-coil Peptides

preprint
submitted on 10.09.2019, 00:22 and posted on 11.09.2019, 15:40 by Alex Buchberger, Chad Simmons, Nour Fahmi, Ronit Freeman, Nicholas Stephanopoulos
DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with orthogonal DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of self-assembled structures through multiple routes, to form dimers and trimers, an alternating copolymer of two different origami bundles, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.

Funding

AFOSR FA9550-17-1-0053

ASU startup funds

History

Email Address of Submitting Author

nstepha1@asu.edu

Institution

Arizona State University

Country

United States

ORCID For Submitting Author

0000-0001-7859-410X

Declaration of Conflict of Interest

None

Exports

Read the published paper

in Journal of the American Chemical Society

Logo branding

Exports