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CC-origami paper - chemRxiv.pdf (8.73 MB)
Hierarchical Assembly of DNA Origami Nanostructures Using Coiled-coil Peptides
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submitted on 10.09.2019 and posted on 11.09.2019by Alex Buchberger, Chad Simmons, Nour Fahmi, Ronit Freeman, Nicholas Stephanopoulos
DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with orthogonal DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of self-assembled structures through multiple routes, to form dimers and trimers, an alternating copolymer of two different origami bundles, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.