These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Cap paper_ChemRxiv.pdf (435.38 kB)
Heterologous Production of Lasso Peptide Capistruin in a Burkholderia Host
Preprints are manuscripts made publicly available before they have been submitted for formal peer review and publication. They might contain new research findings or data. Preprints can be a draft or final version of an author's research but must not have been accepted for publication at the time of submission.
Burkholderia bacteria are an emerging source of natural products with
applications in agriculture and medicine. Heterologous expression of
biosynthetic gene clusters can streamline natural product discovery. However,
production yields with the commonly used Escherichia
coli host are usually low. Following the current paradigm that one host
does not fit all, we aim to develop a Burkholderia
host in order to ultimately tap into the biosynthetic potential of Burkholderia genomes, which can contain
up to 27 biosynthetic gene clusters per genome. Because close phylogenetic
relationship is expected to improve odds of success due to compatible gene
expression and precursor supply, we tested Burkholderia
sp. FERM BP-3421, a non-pathogenic isolate previously used to produce natural
products at industrial scale. We show here that FERM BP-3421 can produce the
model lasso peptide capistruin in yields that are at least 65-fold, and up to
580-fold higher than the previously used E.