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Heterologous Production of Lasso Peptide Capistruin in a Burkholderia Host

preprint
submitted on 25.10.2019 and posted on 29.10.2019 by Sylvia Kunakom, Alessandra Eustaquio
Burkholderia bacteria are an emerging source of natural products with applications in agriculture and medicine. Heterologous expression of biosynthetic gene clusters can streamline natural product discovery. However, production yields with the commonly used Escherichia coli host are usually low. Following the current paradigm that one host does not fit all, we aim to develop a Burkholderia host in order to ultimately tap into the biosynthetic potential of Burkholderia genomes, which can contain up to 27 biosynthetic gene clusters per genome. Because close phylogenetic relationship is expected to improve odds of success due to compatible gene expression and precursor supply, we tested Burkholderia sp. FERM BP-3421, a non-pathogenic isolate previously used to produce natural products at industrial scale. We show here that FERM BP-3421 can produce the model lasso peptide capistruin in yields that are at least 65-fold, and up to 580-fold higher than the previously used E. coli host.

Funding

Institutional Career Development

National Center for Advancing Translational Sciences

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History

Email Address of Submitting Author

ase@uic.edu

Institution

University of Illinois at Chicago

Country

United States

ORCID For Submitting Author

0000-0002-7852-7844

Declaration of Conflict of Interest

No conflict of interest

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