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Halogen bonding: an underestimated player in membrane-ligand interactions

preprint
revised on 30.01.2021, 12:15 and posted on 01.02.2021, 09:41 by Rafael Nunes, Diogo Vila Viçosa, Paulo J. Costa
Halogen bonds (XBs) are noncovalent interactions where halogen atoms act as electrophilic species interacting with Lewis bases. These interactions are relevant in biochemical systems being increasingly explored in drug discovery, mainly to modulate protein–ligand interactions, but are also found in engineered protein or nucleic acid systems. In this work, we report direct evidence for the existence of XBs in the context of biological membrane systems thus expanding the scope of application of these interactions. Indeed, our molecular dynamics simulations show the presence of favorable interactions between halobenzene derivatives and both phosphate or ester oxygen acceptors from model phospholipid bilayers, thus supporting the existence of XB mediated phospholipid–halogen recognition phenomena influencing the membrane insertion profile of the ligands and their orientational preferences. This represents a relevant interaction, previously overlooked, eventually determining the pharmacological or toxicological activity of halogenated compounds and hence with potential implications in drug discovery and development, a place where such species account for a significant part of the chemical space. We also provide insights into a potential role for XBs in water-to membrane insertion of halogenated ligands as XBs are systematically observed during this process. Therefore, our data strongly suggests that, as the ubiquitous hydrogen bond, XBs should be accounted for in the development of membrane partition models.

Funding

IF/00069/2014/CP1216/CT0006

SFRH/BD/116614/2016

UIDB/04046/2020-UIDP/04046/2020

PTDC/QUI-QFI/28455/2017

LISBOA-01-0145- FEDER-028455

UIDB/00100/2020-UIDP/00100/2020

History

Email Address of Submitting Author

pjcosta@fc.ul.pt

Institution

University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute

Country

Portugal

ORCID For Submitting Author

0000-0002-0492-6666

Declaration of Conflict of Interest

The authors declare no conflict of interest

Version Notes

Preprint Ver3

Exports