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H2 as Fuel for Flavin- and H2O2-Dependent Biocatalytic Reactions

preprint
submitted on 05.05.2020 and posted on 06.05.2020 by Ammar Al-Shameri, Sebastian Willot, Caroline Paul, Frank Hollmann, Lars Lauterbach

Herein, we report a novel H2-driven regeneration system for reduced flavin cofactors. Using an O2-tolerant hydrogenase, we achieved NAD(P)H-independent, highly selective, ene-reductase-catalyzed C=C-double bond reductions, monooxygenase-catalyzed epoxidations and peroxygenase-catalyzed hydroxylations. The hydrogenase performed up to 46000 catalytic cycles and product titers of up to 22 mM have been accomplished. Overall, this system bears the promise to power flavin-dependent biocatalytic reactions with H2 in a highly atom efficient manner.

Funding

Deutsche Forschungsgemeinschaft, project number 284111627

Deutsche Forschungsgemeinschaft under Germany´s Excellence Strategy – EXC 2008 – 390540038 – UniSysCat

Fonds der Chemischen Industrie

Netherlands Organization for Scientific Research VICI grant No. 724.014.003

History

Email Address of Submitting Author

lars.lauterbach@tu-berlin.de

Institution

Technische Universität Berlin

Country

Deutschland

ORCID For Submitting Author

0000-0002-6601-6473

Declaration of Conflict of Interest

The authors declare no competing financial interest.

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