ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
chemrxiv_Text.pdf (1.26 MB)

Fucosylated Ubiquitin and Orthogonally Glycosylated Mutant A28C: Conceptually New Ligands for Burkholderia Ambifaria Lectin (BambL)†

preprint
submitted on 12.07.2020 and posted on 14.07.2020 by Cristina Nativi, Sakonwan Kuhaudomlarp, Linda Cerofolini, Sabrina Santarsia, Emilie Gillon, Silvia Fallarini, Grazia Lombardi, Maxime Denis, Stefano Giuntini, Carolina Valori, Marco Fragai, Anne Imberty, Alessandro Dondoni
Ubiquitin as scaffold protein and aryl-α-O-fucoside as determinant to achieve conceptually new ligands with high affinity for Burkholderia ambifaria lectin are described.
Ub mutant A28C which displays a Cys residue in addition to the eight Lys residues was expressed.
The resulting scaffold was orthogonally glycosylated
to display one thio-rhamnose and up to eight residues of aryl-α-O-fucoside.
The glycosylated Ub-based scaffolds have unprecedented immune properties.

History

Email Address of Submitting Author

cristina.nativi@unifi.it

Institution

University of Florence

Country

Italy

ORCID For Submitting Author

0000-0002-6312-3230

Declaration of Conflict of Interest

No conflict of interest

Exports

Logo branding

Exports