Fucosylated Ubiquitin and Orthogonally Glycosylated Mutant A28C: Conceptually New Ligands for Burkholderia Ambifaria Lectin (BambL)†

Ubiquitin as scaffold protein and aryl-α-O-fucoside as determinant to achieve conceptually new ligands with high affinity for Burkholderia ambifaria lectin are described.
Ub mutant A28C which displays a Cys residue in addition to the eight Lys residues was expressed.
The resulting scaffold was orthogonally glycosylated
to display one thio-rhamnose and up to eight residues of aryl-α-O-fucoside.
The glycosylated Ub-based scaffolds have unprecedented immune properties.