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Fluorogenic Protein Probes with Red or Near-Infrared Emission for Genetically Targeted Live-Cell Imaging

preprint
submitted on 13.05.2020 and posted on 14.05.2020 by Sylvestre P. J. T. Bachollet, Cyril Addi, Jean-Maurice Mallet, Blaise Dumat
A series of red-emitting and near-infrared fluorogenic protein probes based on push-pull molecular rotor structures was developed. After characterization of their optical properties using Bovine Serum Albumin as a model protein, they were conjugated to a halogenoalkane ligand in order to target the protein self-labeling tag HaloTag. The interaction with HaloTag was investigated in vitro and then the most promising probes were applied to live-cell imaging in wash-free conditions using fluorogenic and chemogenetic targeting of HaloTag fusion proteins.

Funding

Agence Nationale de la Recherche (ANR-18-CE44-0006)

History

Email Address of Submitting Author

blaise.dumat@ens.psl.eu

Institution

Centre National de la Recherche Scientifique

Country

France

ORCID For Submitting Author

0000-0002-0455-7282

Declaration of Conflict of Interest

There are no conflicts of interest to declare

Version Notes

Version 1.0

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