ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
1/1
0/0

Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming

preprint
revised on 17.09.2018 and posted on 17.09.2018 by Maria del Carmen Marin, Damianos Agathangelou, Yoelvis Orozco-González, Alessio Valentini, Yoshitaka Kato, Rei Abe-Yoshizumi, Hideki Kandori, Ahreum Choi, Kwang-Hwan Jung, Stefan Haacke
The manuscript reports on two mutations of the photo-sensory protein Anabaena Sensory Rhodopsin and how these mutations modify the fluorescence quantum yield with respect to the wild-type protein. Experimental results are presented and explained theoretically on the basis of mixing of the S1 and S2 excited states. This mixing modulated by electrostatic and steric effects, tunes the excited state potential energy surface, and thereby the excited state lifetime and the fluorescence quantum yield.

Funding

ANR-DFG and USIAS (University of Strasbourg), NSF, NIH, MEXT, CREST-JST, NRF

History

Email Address of Submitting Author

haacke@unistra.fr

Institution

University of Strasbourg - CNRS

Country

France

ORCID For Submitting Author

0000-0002-6969-4667

Declaration of Conflict of Interest

None

Version Notes

Version submitted to JACS, under review

Exports