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Flow Imaging Microscopy as a Novel Tool for High-Throughput Evaluation of Elastin-like Polymer Coacervates

preprint
submitted on 21.11.2018 and posted on 21.11.2018 by Laura Marvin, Wynter Paiva, Nicole Gill, Marissa A. Morales, Jeffrey Mark Halpern, James Vesenka, Eva Rose Balog
Biological and bioinspired polymer microparticles have broad biomedical and industrial applications, including drug delivery, tissue engineering, surface modification, environmental remediation, imaging, and sensing. Full realization of the potential of biopolymer microparticles will require methods for rigorous characterization of particle sizes, morphologies, and dynamics, so that researchers may correlate particle characteristics with synthesis methods and desired functions. Toward this end, we evaluated biopolymer microparticles using flow imaging microscopy. This technology is widely used in the biopharmaceutical industry but is not yet well-known among the materials community. Our polymer, a genetically engineered elastin-like polypeptide (ELP), self-assembles into micron-scale coacervates. We performed flow imaging of ELP coacervates using two different instruments, one with a lower size limit of approximately 2 microns, the other with a lower size limit of approximately 300 nanometers. We validated flow imaging results by comparison with dynamic light scattering and atomic force microscopy analyses. We explored the effects of various solvent conditions on ELP coacervate size, morphology, and behavior, such as the dispersion of single particles versus aggregates. We found that flow imaging is a superior tool for rapid and thorough particle analysis of ELP coacervates in solution. We anticipate that researchers studying many types of microscale protein or polymer assemblies will be interested in flow imaging as a tool for quantitative, solution-based characterization.

Funding

NSF 1638893

NSF 1638896

History

Email Address of Submitting Author

ebalog@une.edu

Institution

University of New England

Country

United States

ORCID For Submitting Author

0000-0001-6792-6914

Declaration of Conflict of Interest

no conflict of interest

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