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Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR

preprint
revised on 03.11.2019, 23:21 and posted on 08.11.2019, 16:57 by Martin Jakubec, Espen Bariås, Fedor Kryuchkov, Linda Veka Hjørnevik, Øyvind Halskau
Phospholipid quantification by 31P NMR and lipid separation and detection by LC-MS/MS. Fatty acid quantification using scripted reconstruction of elution profile for each fatty acid associated with each lipid species. The combination of NMR and and MS data is complementary and allows better quantification and more complete description than each approach would allow by itself. Datasets exists for both whole-cell and plasma membrane fraction, and we also describe and document the extraction techniques.

Funding

Cellemembranen som katalyst for skadelig proteinmisfolding

The Research Council of Norway

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NNP - The Norwegian NMR Platform

The Research Council of Norway

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Bergen Research Foundation

History

Email Address of Submitting Author

oyvind.halskau@uib.no

Institution

University of Bergen

Country

Norway

ORCID For Submitting Author

0000-0003-2517-1060

Declaration of Conflict of Interest

Authors declare No Conflicts of Interests

Version Notes

Main Manuscript Online Version 2.0. Supplementary Information Online Version 2.0.

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