Fast Surface Immobilization of Native Proteins through Metal-Free Amino-yne Click Bioconjugation

25 November 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

In this work, we use a spontaneous and catalyst-free amino-yne click bioconjugation to generate activated ethynyl group functionalized surfaces for fast immobilizing native proteins and cells. Biomolecules, such as bovine serum albumin (BSA), human IgG and peptide of C(RGDfK), could be covalently immobilized on the surfaces in as short as 30 min. Notably, the bioactivity of the anchored biomolecules remain intact, which is verified by efficiently capturing target antibodies and cells from the bulk solutions. This strategy represents an alternative for highly efficient surface biofunctionalization.

Keywords

Amino-yne click reaction
surface immobilization
aggregation-induced emission
native biomolecule

Supplementary materials

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