ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
1/1
3 files

Facile Formation of Giant Elastin-like Polypeptide Vesicles as Synthetic Cells

preprint
submitted on 05.02.2021, 00:33 and posted on 05.02.2021, 13:07 by Bineet Sharma, Yutao Ma, Andrew Ferguson, Allen Liu
Creating a suitable compartment for synthetic cells has led the exploration of different cell chassis materials from phospholipids to polymer to protein-polymer conjugates. Currently, the majority of cell-like compartments are made of lipid molecules as the resulting membrane resembles that of a natural cell. However, cell-sized lipid vesicles are prone to physical and chemical stresses and can be unstable in hosting biochemical reactions within. Recently, peptide vesicles that are more robust and stable were developed as a new chassis material for synthetic cells. Here we demonstrate the facile and robust generation of giant peptide vesicles made of elastin-like polypeptides (ELPs) by using an emulsion transfer method. We show that these peptide vesicles can stably encapsulate molecules and can host cell-free expression reactions. We also demonstrate membrane incorporation of another amphiphilic ELP into existing peptide vesicles. Since ELPs are genetically encoded, the approaches presented here provide exciting opportunities to engineer synthetic cell membranes.

Funding

DMR-1939354

DMR-1939463

History

Email Address of Submitting Author

allenliu@umich.edu

Institution

University of Michigan

Country

USA

ORCID For Submitting Author

0000-0002-0309-7018

Declaration of Conflict of Interest

ALF is a co-founder and consultant of Evozyne, LLC and a co-author of US Provisional Patents 62/853,919 and 62/900,420 and International Patent Applications PCT/US2020/035206 and PCT/US20/50466.