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Facile Formation of Giant Elastin-like Polypeptide Vesicles as Synthetic Cells

submitted on 05.02.2021, 00:33 and posted on 05.02.2021, 13:07 by Bineet Sharma, Yutao Ma, Andrew Ferguson, Allen Liu
Creating a suitable compartment for synthetic cells has led the exploration of different cell chassis materials from phospholipids to polymer to protein-polymer conjugates. Currently, the majority of cell-like compartments are made of lipid molecules as the resulting membrane resembles that of a natural cell. However, cell-sized lipid vesicles are prone to physical and chemical stresses and can be unstable in hosting biochemical reactions within. Recently, peptide vesicles that are more robust and stable were developed as a new chassis material for synthetic cells. Here we demonstrate the facile and robust generation of giant peptide vesicles made of elastin-like polypeptides (ELPs) by using an emulsion transfer method. We show that these peptide vesicles can stably encapsulate molecules and can host cell-free expression reactions. We also demonstrate membrane incorporation of another amphiphilic ELP into existing peptide vesicles. Since ELPs are genetically encoded, the approaches presented here provide exciting opportunities to engineer synthetic cell membranes.





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University of Michigan



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Declaration of Conflict of Interest

ALF is a co-founder and consultant of Evozyne, LLC and a co-author of US Provisional Patents 62/853,919 and 62/900,420 and International Patent Applications PCT/US2020/035206 and PCT/US20/50466.