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Evaluation of PC12 Cell Neural Differentiation on Graphene Coated ITO Microchips.pdf (766.45 kB)

Evaluation of PC12 Cell Neural Differentiation on Graphene Coated ITO Microchips

preprint
submitted on 08.10.2020 and posted on 08.10.2020 by Tansu Golcez, Fikri seven, Ozan Karaman, Mustafa Sen
In this study, the impact of graphene on neuronal differentiation of PC12 cells into neuron-like cells was evaluated in conjunction with electrical stimuli. First, an ITO (Indium Tin Oxide) microchip with a certain number of electrodes was fabricated using photolithography and then a chemically synthesized graphene was coated on the microchip. The electrical stimulation was applied through the ITO-microchip. Following optimization of neuronal differentiation conditions, the effect of AC and DC electrical stimulation on both bare and graphene-coated ITO-microchips for neuronal differentiation was investigated. According to the results, it was observed that electrical stimulation with direct current for 30 minutes caused a large degree of neuronal cell differentiation on the graphene coated ITO-microchips. The results were also verified by real-time qPCR.

Funding

Tübitak 3501 (Project no: 215E003)

History

Email Address of Submitting Author

mustafa.sen@ikc.edu.tr

Institution

İzmir Katip Çelebi University

Country

Turkey

ORCID For Submitting Author

https://orcid.org/0000-0002-2421-9184

Declaration of Conflict of Interest

No conflict of interest

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