These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Evaluation of PC12 Cell Neural Differentiation on Graphene Coated ITO Microchips.pdf (766.45 kB)
Evaluation of PC12 Cell Neural Differentiation on Graphene Coated ITO Microchips
Preprints are manuscripts made publicly available before they have been submitted for formal peer review and publication. They might contain new research findings or data. Preprints can be a draft or final version of an author's research but must not have been accepted for publication at the time of submission.
submitted on 08.10.2020 and posted on 08.10.2020by Tansu Golcez, Fikri seven, Ozan Karaman, Mustafa Sen
In this study, the impact of graphene on neuronal
differentiation of PC12 cells into neuron-like cells was evaluated in
conjunction with electrical stimuli. First, an ITO (Indium Tin Oxide) microchip
with a certain number of electrodes was fabricated using photolithography and
then a chemically synthesized graphene was coated on the microchip. The
electrical stimulation was applied through the ITO-microchip. Following
optimization of neuronal differentiation conditions, the effect of AC and DC electrical
stimulation on both bare and graphene-coated ITO-microchips for neuronal
differentiation was investigated. According to the results, it was observed
that electrical stimulation with direct current for 30 minutes caused a large
degree of neuronal cell differentiation on the graphene coated ITO-microchips.
The results were also verified by real-time qPCR.