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Enzymatic Modification of N-Terminal Proline Residues Using Phenol Derivatives

submitted on 29.09.2018, 20:44 and posted on 01.10.2018, 14:45 by Johnathan Maza, Daniel Bader, Lifeng Xiao, Alan Marmelstein, Daniel D. Brauer, Adel M. ElSohly, Matthew J. Smith, Matthew Francis
A convenient enzymatic strategyis reported for the modification of proline residues in the N-terminal positions of proteins. Using a tyrosinase enzyme isolated from Agaricus bisporus(abTYR), phenols and catechols are oxidized to highly reactive o-quinone intermediates that then couple to N-terminal proline residues in high yield. Key advantages of this bioconjugation method include (1) the use of air-stable precursors that can be prepared on large scale if needed, (2) mild reaction conditions, including low temperatures, (3) the targeting of native functional groups that can be introduced readily on most proteins, and (4) the use of molecular oxygen as the sole oxidant. This coupling strategy was successfully demonstrated for the attachment of a variety of phenol-derivatized cargo molecules to a series of protein substrates, including self-assembled viral capsids, enzymes, and a chitin binding domain (CBD). The ability of the CBD to bind to the surfaces of yeast cells was found to be unperturbed by this modification reaction.


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University of California at Berkeley


United States of America

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No conflict of interest


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in Journal of the American Chemical Society