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Enzymatic degradation of poly(butylene succinate) copolyesters synthesized with the use of Candida antarctica lipase B

revised on 20.06.2018, 09:13 and posted on 20.06.2018, 13:06 by Aleksandra Wcislek, Agueda Sonseca Olalla, Andrew McClain, Agnieszka Piegat, Peter Sobolewski, Judit E. Puskas, Miroslawa El Fray

Abstract: Biodegradable polymers are an active area of investigation, particularly ones that can be produced from sustainable, biobased monomers, such as copolymers of poly(butylene succinate) (PBS). In this study, we examine the enzymatic degradation of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers obtained by “green” enzymatic synthesis using lipase B from Candida antarctica (CALB). The copolymers differed in their hard to soft segments ratio, from 70:30 to 50:50 wt.%. Enzymatic degradation was carried out on electrospun membranes (scaffolds) and compression-moulded films using lipase fromPseudomomas cepacia. Poly(e-caprolactone) (PCL) was used as a reference aliphatic polyester. The degradation process was monitored gravimetrically via water uptake and mass loss. After 24 days, approx. 40% mass loss was observed for fibrous materials prepared from PBS-DLS 70:30 copolymer, as compared to approx. 10% mass loss for PBS-DLS 50:50. Infrared spectroscopy (FTIR) and SEC analysis were used to examine changes in chemical structure. Differential scanning calorimetry (DSC) and scanning light microscopy (LSM) revealed changes in degree of crystallinity, and changes in surface morphology, consistent with a surface erosion mechanism. We conclude that the obtained copolymers are suitable for tissue engineering applications thanks to tuneable degradation and lack of acidification during breakdown.


National Science Centre, Poland, under the HARMONIA program under agreement No.UMO-2014/14/M/ST00610.


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Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology, Szczecin



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Declaration of Conflict of Interest

no conflict of interest

Version Notes

Corrected Figure numbering (were off by 1 after #2)