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Enhanced-Fluorescence of a Dye on DNA- assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy

preprint
submitted on 22.12.2017 and posted on 27.12.2017 by Pedro M. R. Paulo, David Botequim, Agnieszka Jóskowiak, Sofia Martins, Duarte M. F. Prazeres, Peter Zijlstra, Sílvia M. B. Costa

We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon- enhanced fluorescence of this dye using dimers of 80 nm particles was measured at single molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and, in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from non-enhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps.

History

Topic

  • Photochemistry (Physical Chem.)

Email Address of Submitting Author

pedro.m.paulo@tecnico.ulisboa.pt

Institution

Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa

Country

Portugal

ORCID For Submitting Author

0000-0002-8001-4810

Declaration of Conflict of Interest

The authors declare no conflict of interest

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