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Enhanced-Fluorescence of a Dye on DNA- assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy

preprint
submitted on 22.12.2017, 00:17 and posted on 27.12.2017, 13:58 by Pedro M. R. Paulo, David Botequim, Agnieszka Jóskowiak, Sofia Martins, Duarte M. F. Prazeres, Peter Zijlstra, Sílvia M. B. Costa

We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon- enhanced fluorescence of this dye using dimers of 80 nm particles was measured at single molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and, in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from non-enhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps.

History

Topic

  • Photochemistry (Physical Chem.)

Email Address of Submitting Author

pedro.m.paulo@tecnico.ulisboa.pt

Institution

Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa

Country

Portugal

ORCID For Submitting Author

0000-0002-8001-4810

Declaration of Conflict of Interest

The authors declare no conflict of interest

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