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Formoterol manuscript.pdf (622.48 kB)

Enantioselective Chromatographic Analysis of Formoterol Fumarate using Chiral Mobile Phase Additives and Achiral Core-Shell Column

preprint
submitted on 01.07.2020, 12:39 and posted on 02.07.2020, 11:44 by Pranav Pathak, Krishnapriya Mohanraj

A simple, robust, cost-effective, and rapid RP-HPLC method was developed for the separation of enantiomers of formoterol. The separation was achieved by the chiral mobile phase additive technique on an achiral column. Formoterol is a bronchodilator that consists of 50:50 S, S-formoterol and R, R-formoterol. The bronchodilator activity is attributed to R, R-formoterol. Hence, it is important to develop a method to separate the enantiomers of formoterol. Various factors affecting enantiomeric resolution were investigated and optimized. The enantiomers of formoterol were successfully separated with a resolution of 2.57 with a run-time of 9 minutes. The method was validated in accordance with ICH guidelines. The validated method was successfully applied to the marketed formulation of arformoterol.

Funding

Department of Science and Technology (DST-DPRP: Project sanction number: VI-D&P/552/2016-17/TDT (G) dated 07.03.2017)

History

Email Address of Submitting Author

psp1112@gmail.com

Institution

Bombay College of Pharmacy

Country

India

ORCID For Submitting Author

0000-0002-2576-8265

Declaration of Conflict of Interest

No conflict of Interest

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