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Enabling Indium Channels for Mass Cytometry by Using Reinforced Cyclambased Chelating Polylysine

preprint
submitted on 18.04.2020 and posted on 21.04.2020 by Laura Grenier, Maryline Beyler, Taunia L. L. Closson, Nick Zabinyakov, Alexandre Bouzekri, Yefeng Zhang, Jothir Mayanantham Pichaandi, Mitchell A. Winnik, Peng Liu, olga ornatsky, Vladimir Baranov, Raphael Tripier
A metal containing polymer (MCP) based on a polylysine functionalized by In(III) chelates was synthesized. The chelator is based on a constrained dipicolinate cyclam that forms a highly inert In(III) complex. The MCP was conjugated to anti CD20 antibody using the very strong neutravidin/biotin interaction. Two cell lines, one expressing CD20 the other not, were stained with the modified antibody and analysed by mass cytometry using the In-115 channel. The results showed a specific antigen-antibody recognition and images by mass cytometry imaging could be obtained thanks to In-115 detection. Finally, overtime stability tests of the bioconjugate as well as multiplex experiments using the In-115 channel underline the high potentiel of this new In based MCP.

History

Email Address of Submitting Author

maryline.beyler@univ-brest.fr

Institution

University of Brest

Country

France

ORCID For Submitting Author

0000-0002-6540-970X

Declaration of Conflict of Interest

No conflict of interest

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