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art-ChemRxiv_Grenier_17_04.pdf (1.98 MB)

Enabling Indium Channels for Mass Cytometry by Using Reinforced Cyclambased Chelating Polylysine

preprint
submitted on 18.04.2020, 08:06 and posted on 21.04.2020, 08:52 by Laura Grenier, Maryline Beyler, Taunia L. L. Closson, Nick Zabinyakov, Alexandre Bouzekri, Yefeng Zhang, Jothir Mayanantham Pichaandi, Mitchell A. Winnik, Peng Liu, olga ornatsky, Vladimir Baranov, Raphael Tripier
A metal containing polymer (MCP) based on a polylysine functionalized by In(III) chelates was synthesized. The chelator is based on a constrained dipicolinate cyclam that forms a highly inert In(III) complex. The MCP was conjugated to anti CD20 antibody using the very strong neutravidin/biotin interaction. Two cell lines, one expressing CD20 the other not, were stained with the modified antibody and analysed by mass cytometry using the In-115 channel. The results showed a specific antigen-antibody recognition and images by mass cytometry imaging could be obtained thanks to In-115 detection. Finally, overtime stability tests of the bioconjugate as well as multiplex experiments using the In-115 channel underline the high potentiel of this new In based MCP.

History

Email Address of Submitting Author

maryline.beyler@univ-brest.fr

Institution

University of Brest

Country

France

ORCID For Submitting Author

0000-0002-6540-970X

Declaration of Conflict of Interest

No conflict of interest

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