ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
IgG for ChemRXIV_Paper Final.pdf (744.45 kB)

Electrochemical-Based Serological Detection of Bovine Immunoglobulin G in Calves

preprint
submitted on 03.02.2020, 15:05 and posted on 04.02.2020, 10:34 by Caoimhe Robinson, Niamh Creedon, Riona sayers, emer kennedy, Alan O'Riordan
Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus, in utero. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 24 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficent detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves’ vitality and survival rate

Funding

16/RC/3835

17/RD/US-ROI/56

History

Email Address of Submitting Author

alan.oriordan@tyndall.ie

Institution

Tyndall National Institute - University College COrk

Country

Ireland

ORCID For Submitting Author

0000-0002-7554-1536

Declaration of Conflict of Interest

none

Exports

Logo branding

Exports