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Effects of Single α-to-β Residue Replacements on Recognition of an Extended Segment in a Viral Fusion Protein

preprint
submitted on 27.04.2020 and posted on 28.04.2020 by Victor Outlaw, Dale F. Kreitler, Debora Stelitano, Matteo Porotto, Anne Moscona, Samuel H. Gellman
Partial replacement of α-amino acid residues with β-amino acid residues has been established as a strategy for preserving target-engagement by helix-forming polypeptides while suppressing susceptibility to proteolysis. The impact of β-residue incorporation within polypeptides that adopt less regular conformations, however, has received less attention. The HRC domains of fusion glycoproteins from pathogenic paramyxoviruses contain a segment that must adopt an extended conformation in order to engage the HRN domain in the post-fusion state and drive merger of the viral envelope with a target cell membrane. Here we examine the impact of single α-to-β substi-tutions within this extended N-terminal segment of the engineered HRC peptide VIQKI. Stabilities of helix-bundles formed with a native viral HRN have been evaluated, the structures of five helix-bundles have been determined, and antiviral efficacies have been measured. Many sites within the extended segment show functional tolerance of α-to-β substitution. These results offer a basis for future develop-ment of protease-resistant inhibitors of paramyxovirus infection.

Funding

Engineering protease-resistant alpha-beta peptides for broad-spectrum antivirals

National Institute of Allergy and Infectious Diseases

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Development of novel endosome-targeted Ebola virus entry inhibitors as antiviral agents

National Institute of Allergy and Infectious Diseases

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Structure-Guided Design of Peptidomimetic Inhibitors of Paramyxoviral Fusion

National Institute of General Medical Sciences

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History

Email Address of Submitting Author

voutlaw@wisc.edu

Institution

University of Wisconsin-Madison

Country

United States

ORCID For Submitting Author

0000-0001-7054-4204

Declaration of Conflict of Interest

No conflict of interest

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