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20.05.07_Joseph Srinivasan, S et al, 2020, E coli hydrogenase flavin recycling unlinked.pdf (1.89 MB)
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E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme

preprint
revised on 07.05.2020 and posted on 08.05.2020 by Shiny Joseph Srinivasan, Sarah Cleary, Caroline Paul, Miguel A. Ramirez, Kylie Vincent

Robust [NiFe] hydrogenase 1 (Hyd1) from Escherichia coli is shown to have non-native, H2-dependent activity for FMN and FAD reduction, and to function as a promising recycling system for FMNH2 supply to flavoenzymes for chemical synthesis, giving a total turnover number over 10 thousand when coupled with an Old Yellow Enzyme ene reductase.

Funding

BiocatSusChem ERC-2018-CoG 819580

BBSRC, BB/R018413/1

EPSRC, IB Catalyst EP/N013514/1

History

Email Address of Submitting Author

sarah.cleary@chem.ox.ac.uk

Institution

University of Oxford

Country

United Kingdom

ORCID For Submitting Author

0000-0001-8876-1696

Declaration of Conflict of Interest

The authors declare no conflict of interest

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