These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
realtime_final.pdf (307.64 kB)
Dynamic Real-Time Magnetic Resonance at Very Low Magnetic Fields
Preprints are manuscripts made publicly available before they have been submitted for formal peer review and publication. They might contain new research findings or data. Preprints can be a draft or final version of an author's research but must not have been accepted for publication at the time of submission.
submitted on 03.09.2020 and posted on 03.09.2020by Sergey Korchak, Anil P. Jagtap, Stefan Gloeggler
The phenomenon of nuclear magnetic resonance (NMR) is widely applied in biomedical and
biological science to study structures and dynamics of proteins and their reactions. Despite
its impact, NMR is an inherently insensitive phenomenon and has driven the field to construct
spectrometers with increasingly higher magnetic fields leading to more detection sensitivity.
Here, we are demonstrating that enzymatic reactions can be followed in real-time at millitesla
fields, 1000-fold lower than state-of-the-art spectrometers. This requires signal-enhancing
samples via hyperpolarization. Within seconds, we have enhanced the signals of 2-
13Cpyruvate, an important metabolite to probe cancer metabolism, in 22 mM concentrations (up
to 10.1%±0.1% polarization) and show that such a large signal allows for the real-time
detection of enzymatic conversion of pyruvate to lactate. This development paves the
pathways for biological studies in portable and affordable NMR systems with a potential for