Dynamic Formation of Liquid Droplets Triggered by Sequential Enzymatic Reactions

04 March 2020, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

A model system was developed that dynamically generates two different liquid droplets via liquid–liquid phase separation coupled with a sequential glycolytic reaction. The sequential two-enzyme reaction triggers the formation/dissolution of the liquid droplets. The droplets, in turn, compartmentalize each enzymatic step and generate feedback to accelerate the overall reaction.

Keywords

liquid-liquid phase separation
sequential enzymatic reaction

Supplementary materials

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