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Dynamic Formation of Liquid Droplets Triggered by Sequential Enzymatic Reactions

preprint
submitted on 04.03.2020 and posted on 04.03.2020 by Tomoto Ura, Shunsuke Tomita, Kentaro Shiraki

A model system was developed that dynamically generates two different liquid droplets via liquid–liquid phase separation coupled with a sequential glycolytic reaction. The sequential two-enzyme reaction triggers the formation/dissolution of the liquid droplets. The droplets, in turn, compartmentalize each enzymatic step and generate feedback to accelerate the overall reaction.

Funding

JSPS KAKENHI (grant number 18H02383 and 19K22377)

History

Email Address of Submitting Author

shiraki@bk.tsukuba.ac.jp

Institution

Faculty of Pure and Applied Sciences, University of Tsukuba

Country

Japan

ORCID For Submitting Author

0000-0003-3438-4076

Declaration of Conflict of Interest

The authors declare no competing financial interest.

Version Notes

First version

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