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HRR_MPIs_chemrxiv_210129.pdf (5.88 MB)

Detection of Metabolite-Protein Interactions in Complex Biological Samples by High-Resolution Relaxometry: Towards Interactomics by NMR

preprint
revised on 29.01.2021, 22:33 and posted on 01.02.2021, 09:17 by Ziqing Wang, Veronica Ghini, Pavel Kaderavek, Simone Pisano, Milan Zachrdla, Philippe Pelupessy, Morgan Kazmierczak, Thorsten Marquardsen, Jean-Max Tyburn, Guillaume Bouvignies, Giacomo Parigi, Claudio Luchinat, Fabien Ferrage
In this manuscript, we introduce an NMR method to investigate the interaction of metabolites and other small molecules with proteins in biological fluids. Our approach, based on high-resolution relaxometry identifies the interaction of small molecules with proteins in blood serum and is used for the quantitative analysis of binding competition in situ.

Funding

European Research Council (ERC) Grant agreement 825404 (RELAX-MAX)

FET-Open Grant agreement 899683 (HIRES-MULTIDYN)

EURELAX COST Action CA15209

AIRC fellowship (Italy)

Fondazione Cassa di Risparmio di Firenze

History

Email Address of Submitting Author

Fabien.Ferrage@ens.psl.eu

Institution

Ecole Normale Supérieure - PSL

Country

France

ORCID For Submitting Author

0000-0002-4738-1723

Declaration of Conflict of Interest

J.-M. T. and T. M. are employees of Bruker Biospin.