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Detection Limit for Optically Sensing Specific Protein Interactions in Free-solution

preprint
submitted on 01.12.2017 and posted on 01.12.2017 by Harish Sasikumar, Manoj M. Varma
Optical molecular sensing techniques are often limited by the refractive index change associated with the probed interactions. In this work, we present a closed form analytical model to estimate the magnitude of optical refractive index change arising from protein-protein interactions. The model, based on the Maxwell Garnett effective medium theory and first order chemical kinetics serves as a general framework for estimating the detection limits of optical sensing of molecular interactions. The model is applicable to situations where one interacting species is immobilized to a surface, as commonly done, or to emerging techniques such as Back-Scattering Interferometry (BSI) where both interacting species are un-tethered. Our findings from this model point to the strong role of as yet unidentified factors in the origin of the BSI signal resulting in significant deviation from linear optical response.

History

Topic

  • Analytical Chemistry

Email Address of Submitting Author

harishsk@iisc.ac.in

Email Address(es) for Other Author(s)

mvarma@iisc.ac.in

Institution

Indian Institute of Science

Country

India

ORCID For Submitting Author

0000-0002-5816-4003

Declaration of Conflict of Interest

The authors declare no competing financial interest.

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