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ChemRxiv-Usman et al 2020.pdf (2.2 MB)

Design of a Well-Defined Poly(Dimethylsiloxane)-Based Microbial Nanoculture System

preprint
submitted on 20.10.2020, 01:47 and posted on 20.10.2020, 12:41 by Huda Usman, Shanna-Leigh Davidson, Nithil H. Manimaran, Jenna T. Nguyen, Aïssatou Bah, Rishabh Seth, Eric Beckman, Tagbo Niepa

Organosilanes contain hydrocarbon-like backbones, allowing them to react with silicone-based agents in the presence of a catalyst and polymerize into membranes with tunable transport and mechanical properties. Owing to their high hydrophobicity, Poly(dimethylsiloxane) (PDMS) membranes, and more particularly, Sylgard® 184, have been used for applications including drug delivery, gas separation, and microfluidics fabrication. However, the undefined composition of the material and its ability to leach out uncured oligomers make its functionalization and usage challenging for many biological applications. This article presents the design of a novel culture system generated using PDMS-based membranes to study microbial dynamics. The microbial culture system that is referred to as “nanoculture” serves to encapsulate and grow microbes in semipermeable membranes. The mechanical properties of the membranes are reinforced through osmotic annealing, which enable the nanocultures to withstand high shear stress similar to environmental conditions while maintaining transport properties essential to microbial communication and growth. The present study lays the foundation for a novel microbial culture system that would enable the cultivation of microorganisms in environments other than laboratory conditions.

Funding

University of Pittsburgh StartUp Funds

History

Email Address of Submitting Author

tniepa@pitt.edu

Institution

University of Pittsburgh

Country

USA

ORCID For Submitting Author

0000-0002-3018-5419

Declaration of Conflict of Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Version Notes

Pre-proof Version Submitted for Publication

Exports