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Demystifying and Unravelling the Factual Molecular Structure of the Biopolymer Sporopollenin

preprint
revised on 18.11.2019 and posted on 27.11.2019 by Abanoub Mikhael, Kristina Jurcic, Celine Schneider, David carr, Gregory L. Fisher, Travis D. Fridgen, Alberto Diego-Taboada, Grahame Mackenzie, JOSEPH BANOUB

Sporopollenin is a natural, highly cross-linked biopolymer composed of carbon, hydrogen, and oxygen, which forms the outer wall of pollen grains. Sporopollenin is resilient to chemical degradation. Because of this stability, its exact chemical structure and the biochemical pathways involved in its biosynthesis remains a mystery and unresolved.

We have identified and characterized the molecular structure of the clean, intact sporopollenin using soft ionization mass spectrometric and nuclear magnetic resonance techniques. These analyses showed that sporopollenin contained a poly(hydroxyacid) dendrimer-like network, which accounted for the sporopollenin empirical formula. In addition, the identified hydroxy acid monomers contained a beta diketone moiety, which most probably accounts for the known antioxidant activity of sporopollenin. Moreover, our elucidation studies allowed us to identify a unique circular polyhydroxylated tetraketide polymer. This polymer acted as the rigid backbone on which the poly(hydroxyacid) network can be built, forming the scaffold of the spherical sporopollenin exine.


History

Email Address of Submitting Author

banoubjo@dfo-mpo.gc.ca

Institution

Fisheries and Oceans Canada, and Memorial University

Country

Canada

ORCID For Submitting Author

0000-0002-6205-7753

Declaration of Conflict of Interest

No Conflicts

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