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Vercruysse et al._dark or light colored melanins_Chemistry Preprint Server.pdf (1.04 MB)

Dark- or Light-Colored Melanins: Generating Pigments Using Fe2+ and H2O2

submitted on 29.11.2017 and posted on 01.12.2017 by Koen Vercruysse, Shelby Russell, Juan Knight, Najwa Stewart, Nicole Wilson, Nahfisa Richardson
We have studied the formation of melanin-like pigments from catechol or pyrogallol and a wide range of other phenolic compounds using Fe2+ and H2O2. Combining UV_Vis spectroscopic measurements and size-exclusion chromatography analyses we evaluated the impact of the intensity of the oxidation reaction by varying the concentration of H2O2 present in the reaction mixtures. All compounds tested, except tyrosine, reacted readily leading to mixtures that were black, brown or yellow-orange in color. For many compounds tested, the use of increasing concentrations of H2O2 resulted in either precipitation of the pigment or the formation of a soluble, lighter-colored pigment. With catechol or pyrogallol as model compounds, and using different concentrations of H2O2, several materials were synthesized, purified and dried. The physic-chemical properties of these materials were compared to the properties of melanin-like pigments synthesized from the same precursors using air-oxidation in an alkaline environment. For both precursors, a distinct chemical change, as judged from FT-IR spectroscopy, was introduced in the melanin structures when using H2O2 as the oxidizing agent and the relative intensity of this distinct signal strengthened with increasing concentration of H2O2 used in the reaction. In general, our results suggest that depending on the precursor molecule and the intensity of the oxidizing reaction conditions involved, light- or dark-colored melanin-like pigments can be generated. This may be an important factor when evaluating the visible outlook of histological or archeological specimens: the presence of a lighter color or the absence of a dark color may not necessarily mean the absence of melanin-like biomolecules.



  • Biochemistry

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Tennessee State University



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