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Yao 2020 Lys acylation 23 combined subm.pdf (3.58 MB)

DNAzymes for Amine and Peptide Lysine Acylation

preprint
submitted on 25.09.2020, 21:06 and posted on 28.09.2020, 13:36 by Tianjiong Yao, Jack Przybyla, Peter Yeh, Austin Woodard, Hannah Nilsson, Benjamin Brandsen, Scott Silverman
DNAzymes were previously identified by in vitro selection for a variety of chemical reactions, including several biologically relevant peptide modifications. However, finding DNAzymes for peptide lysine acylation is a substantial challenge. By using suitably reactive aryl ester acyl donors as the electrophiles, here we used in vitro selection to identify DNAzymes that acylate amines, including lysine side chains of DNA-anchored peptides. Some of the DNAzymes can transfer a small glutaryl group to an amino group. These results expand the scope of DNAzyme catalysis and suggest the future broader applicability of DNAzymes for sequence-selective lysine acylation of peptide and protein substrates.

Funding

NIH GM065966

History

Email Address of Submitting Author

sks@illinois.edu

Institution

University of Illinois at Urbana-Champaign

Country

USA

ORCID For Submitting Author

0000-0001-8166-3460

Declaration of Conflict of Interest

No conflict of interest

Version Notes

Manuscript version 1

Exports