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DNA/TNA Mesoscopic Modeling of Melting Temperatures Suggest Weaker Hydrogen Bonding of CG than in DNA/RNA

preprint
submitted on 03.03.2020, 22:39 and posted on 04.03.2020, 12:55 by Maria Izabel Muniz, Hershel Lackey, Jennifer Heemstra, Gerald Weber
TNA/DNA hybrids share several similarities to RNA/DNA, such as the tendency to form A-type helices and a strong dependency of their thermodynamic properties on purine/pyrimidine ratio. However, unlike RNA/DNA, not much is known about the base-pair properties of TNA. Here, we use a mesoscopic analysis of measured melting temperatures to obtain an estimate of hydrogen bonds and stacking interactions. Our results reveal that the AT base pairs in TNA/DNA have nearly identical hydrogen bond strengths than their counterparts in RNA/DNA, but surprisingly CG turned out to be much weaker despite similar stability.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação de Amparo à Pesquisa do Estado de Minas Gerais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

National Science Foundation CHE-1904885

Air Force Institute of Technology Civilian Institute Program

History

Email Address of Submitting Author

gweberbh@gmail.com

Institution

Universidade Federal de Minas Gerais

Country

Brazil

ORCID For Submitting Author

0000-0002-2935-1571

Declaration of Conflict of Interest

no conflict of interest

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