These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
DNA Minor Groove Binding with Bisbenzamidine-Ru(II) Complexes
preprintsubmitted on 13.05.2019, 16:12 and posted on 15.05.2019, 16:04 by Mateo I. Sánchez, Gustavo Rama, Renata Calo, Kübra Ucar, Per Lincoln, Miguel Vázquez López, Manuel Melle-Franco, José Luis Mascareñas, M. Eugenio Vázquez
We report the first Ru(II) coordination compounds that interact with DNA through a canonical minor groove insertion mode and with selectivity for A/T rich sites. This was made possible by integrating a bis‑benzamidine minor groove DNA-binding agent with a ruthenium(II) complex. Importantly, one of the enantiomers (Δ‑[Ru(bpy)2b4bpy]2+, Δ‑4Ru) shows a considerably higher DNA affinity than the parent organic ligand and than the other enantiomer, particularly for the AATT sequence, while the other enantiomer preferentially targets long AAATTT sites with overall lower affinity. Finally, we demonstrate that the photophysical properties of these new binders can be exploited for DNA cleavage using visible light.