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DNA Microviscosity Converts Ruthenium Polypyridyl Complexes to Effective Photosensitizers

preprint
submitted on 29.01.2020 and posted on 31.01.2020 by Prashant Kumar, Falguni Chandra, Paltan Laha, Srikanta Patra, Apurba Koner

A unique radiative decay engineering strategy using DNA microviscosity for the generation of ruthenium polypyridyl complex (RPCs) mediated singlet oxygen for selective damage of DNA and killing cancer cells is reported. This investigation also demonstarte the effect of light-driven RPCs on bacterial growth arrest, through DNA nick, and differential localization in cancer and non-cancer cells. Moreover, upon binding with DNA, RPCs experience high local microviscosity, which causes significant enhancement of the excited state lifetime and thus generates singlet oxygen. The visible-light-triggered singlet-oxygen efficiently produce nick in DNA and inhibits bacterial growth. RPCs also localize inside the nucleus of the cancer cell and in the vicinity of the nuclear membrane of non-cancerous cells, confirmed by live-cell confocal microscopy. The results provide a facile platform for the novel antibiotic intended discovery combined with cancer therapy.

Funding

Science and Engineering Research Board, INDIA, Grant no: IMP/2018/000898

Science and Engineering Research Board, INDIA, Grant no: CRG/2018/000173

History

Email Address of Submitting Author

akoner@iiserb.ac.in

Institution

IISER Bhopal and IIT Bhubaneswar

Country

INDIA

ORCID For Submitting Author

0000-0002-8891-416X

Declaration of Conflict of Interest

We declare no conflict of interest.

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