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DNA Detection Using Programmed Bilayer Nanopores

preprint
revised on 30.08.2018 and posted on 03.09.2018 by Ramkumar Balasubramanian, Sohini Pal, Himanshu Joshi, Banani Chakraborty, Akshay Naik, Manoj Varma, Prabal K. Maiti

Pore-functionalization has been explored by several groups as a strategy to control DNA translocation through solid-state nanopores. Here we present a hybrid nanopore system consisting of single-layer graphene and a DNA origami layer to achieve base-selective control of DNA translocation rate through aligned nanopores of the two layers. This is achieved by incorporating unpaired dangling bases called overhangs to the origami near the pore region. Molecular dynamics simulations were used to optimize the design of the origami nanopore and the overhangs. Specifically, we considered the influence of the number and spatial distribution of overhangs on translocation times. The simulations revealed that specific interactions between the overhangs and the translocating single stranded DNA resulted in base-specific residence times.

History

Email Address of Submitting Author

ramkumarb@iisc.ac.in

Institution

Indian Institute of Science

Country

India

ORCID For Submitting Author

0000-0001-8775-1832

Declaration of Conflict of Interest

No conflict of interest

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