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Cross-Validation of ELISA and a Portable Surface Plasmon Resonance Instrument for IgG Antibodies Serology with SARS-CoV-2 Positive Individuals

preprint
submitted on 01.04.2021, 10:08 and posted on 02.04.2021, 06:27 by Abdelhadi Djaileb, Maryam Hojjat Jodaylami, Julien Coutu, Pierre Ricard, Mathieu Lamarre, lea rochet, Stella Cellier-Goetghebeur, devin macauley, Benjamin Charron, Vincent Thibault, Keisean Stevenson, Simon Forest, Ludovic S. Live, Nanouk Abonnenc, Anthony Guedon, Patrik Quessy, Jean-Francois Lemay, Omar Farnos, Amine A. Kamen, Matthew Stuible, Christian Gervais, Yves Durocher, François Cholette, Christine Mesa, John Kim, Marie-Pierre Cayer, Marie-Joëlle De Grandmont, Danny Brouard, Sylvie Trottier, Denis Boudreau, Joelle N. Pelletier, Jean-Francois Masson
We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for the detection of nucleocapsid and spike antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma and dried blood spots (DBS). When exposed to SARS-CoV-2 or a vaccine against SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the fraction of the population potentially immunized against SARS-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with various sources of SARS-CoV-2 recombinant proteins expressed in different cell lines detected human anti-SARS-CoV-2 IgG in the nanomolar range. Nucleocapsid expressed in different cell lines did not significantly change the sensitivity of the assays, whereas the use of a CHO cell line to express spike ectodomain led to excellent performance. This bioassay was performed on a portable SPR instrument capable of measuring 4 biological samples within 30 minutes of sample/sensor contact and the chip could be regenerated at least 9 times. Multi-site validation was then performed with in-house and commercial ELISA, which revealed excellent cross-correlations with Pearson’s coefficients exceeding 0.85 in all cases, for measurements in DBS and plasma. This strategy paves the way to point-of-care and rapid testing for antibodies in the context of viral infection and vaccine efficacy monitoring.

Funding

Canadian Institutes of Health Research

Natural Sciences and Engineering Research Council of Canada (NSERC)

Natural Sciences and Engineering Research Council

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College and Community Innovation Program – Applied Research Rapid Response to COVID-19

Canadian Foundation for Innovation

History

Email Address of Submitting Author

jf.masson@umontreal.ca

Institution

Université de Montréal

Country

Canada

ORCID For Submitting Author

0000-0002-0101-0468

Declaration of Conflict of Interest

Jean-Francois Masson, Joelle N. Pelletier and Ludovic S. Live have financial interest in Affinité Instruments.

Version Notes

version 1. April 1st 2021

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