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Cross-Peaks in Simple 2D NMR Experiments from Chemical Exchange of Transverse Magnetization

preprint
submitted on 10.03.2019 and posted on 11.03.2019 by Chris Waudby, Tom Frenkiel, John Christodoulou
Two-dimensional correlation measurements such as COSY, NOESY, HMQC and HSQC experiments are central to small molecule and biomolecular NMR spectroscopy, and commonly form the basis of more complex experiments designed to study chemical exchange occurring during additional mixing periods. However, exchange occurring during chemical shift evolution periods can also influence the appearance of such spectra. While this is often exploited through one-dimensional lineshape analysis ('dynamic NMR'), the analysis of exchange across multiple chemical shift evolution periods has received less attention. Here we report that chemical exchange-induced cross-peaks can arise in even the simplest two-dimensional NMR experiments. These cross-peaks can have highly distorted phases that contain rich information about the underlying exchange process. The quantitative analysis of such peaks, from a single 2D spectrum, can provide a highly accurate characterization of underlying exchange processes.

Funding

Integrative structural biology of protein folding during biosynthesis on the ribosome

Wellcome Trust

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NMR Centre

Cancer Research UK

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History

Email Address of Submitting Author

c.waudby@ucl.ac.uk

Institution

UCL

Country

UK

ORCID For Submitting Author

0000-0001-7810-3753

Declaration of Conflict of Interest

No conflict of interest

Licence

Exports