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TEVp_Manuscript_24Oct2019.pdf (1.46 MB)

Covalent and Non-Covalent Strategies for the Immobilization of Tobacco Etch Virus (TEV) Protease on Superparamagnetic Nanoparticles

preprint
submitted on 13.12.2019, 13:35 and posted on 19.12.2019, 13:03 by Jessica L. Norris, Tulsi Patel, Anvesh K. R. Dasari, Thomas A. Cope, Kwang Hun Lim, Robert Hughes
TEV protease fusion proteins were prepared and immobilized on superparamagnetic nanoparticles (SPIONs). Non-covalent (avidin-biotin) and covalent (HALOtag-chloroalkane) strategies were explored for TEV immobilization. HALOtag immobilized TEV protease provided enhance performance in both enzyme retention and reusability. SPION-immobilized proteases could be a convenient and cost-saving tool in the life sciences

History

Email Address of Submitting Author

hughesr16@ecu.edu

Institution

East Carolina University

Country

United States

ORCID For Submitting Author

0000-0002-4465-4212

Declaration of Conflict of Interest

No conflict of interest

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