ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
TEVp_Manuscript_24Oct2019.pdf (1.46 MB)
0/0

Covalent and Non-Covalent Strategies for the Immobilization of Tobacco Etch Virus (TEV) Protease on Superparamagnetic Nanoparticles

preprint
submitted on 13.12.2019 and posted on 19.12.2019 by Jessica L. Norris, Tulsi Patel, Anvesh K. R. Dasari, Thomas A. Cope, Kwang Hun Lim, Robert Hughes
TEV protease fusion proteins were prepared and immobilized on superparamagnetic nanoparticles (SPIONs). Non-covalent (avidin-biotin) and covalent (HALOtag-chloroalkane) strategies were explored for TEV immobilization. HALOtag immobilized TEV protease provided enhance performance in both enzyme retention and reusability. SPION-immobilized proteases could be a convenient and cost-saving tool in the life sciences

History

Email Address of Submitting Author

hughesr16@ecu.edu

Institution

East Carolina University

Country

United States

ORCID For Submitting Author

0000-0002-4465-4212

Declaration of Conflict of Interest

No conflict of interest

Exports