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Coupling 193 nm Ultraviolet Photodissociation and Ion Mobility for Sequence Characterization of Conformationally-Selected Peptides

preprint
submitted on 09.07.2020 and posted on 10.07.2020 by Alyssa Stiving, Sophie Harvey, Benjamin J. Jones, Bruno Bellina, Jeffery Brown, Perdita E. Barran, Vicki Wysocki
Ultraviolet photodissociation (UVPD) has emerged as a useful technique for characterizing peptide, protein, and protein complex primary and secondary structure. We present here the modification of a commercial ion mobility (IM) mass spectrometer to incorporate 193 nm UVPD following IM separation, enabling IM-UVPD to fragment species selected by both their m/z (via quadrupole selection) and conformation (mobility-selection).

Funding

Resource for Native Mass Spectrometry Guided Structural Biology

National Institute of General Medical Sciences

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History

Email Address of Submitting Author

wysocki.11@osu.edu

Institution

The Ohio State University

Country

United States

ORCID For Submitting Author

0000-0002-9334-1212

Declaration of Conflict of Interest

Jeffery Brown is an employee of Waters Corporation, which manufactures and sells Synapt G2-S instruments.

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