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Coupling 193 nm Ultraviolet Photodissociation and Ion Mobility for Sequence Characterization of Conformationally-Selected Peptides

preprint
submitted on 09.07.2020, 22:47 and posted on 10.07.2020, 12:51 by Alyssa Stiving, Sophie Harvey, Benjamin J. Jones, Bruno Bellina, Jeffery Brown, Perdita E. Barran, Vicki Wysocki
Ultraviolet photodissociation (UVPD) has emerged as a useful technique for characterizing peptide, protein, and protein complex primary and secondary structure. We present here the modification of a commercial ion mobility (IM) mass spectrometer to incorporate 193 nm UVPD following IM separation, enabling IM-UVPD to fragment species selected by both their m/z (via quadrupole selection) and conformation (mobility-selection).

Funding

Resource for Native Mass Spectrometry Guided Structural Biology

National Institute of General Medical Sciences

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History

Email Address of Submitting Author

wysocki.11@osu.edu

Institution

The Ohio State University

Country

United States

ORCID For Submitting Author

0000-0002-9334-1212

Declaration of Conflict of Interest

Jeffery Brown is an employee of Waters Corporation, which manufactures and sells Synapt G2-S instruments.

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