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Chemrxiv_Carraro_etal.pdf (535.92 kB)

Continuous Flow Synthesis of ZIF-8 Biocomposites with Tuneable Particle Size

submitted on 17.01.2020, 16:03 and posted on 21.01.2020, 07:25 by Francesco Carraro, Jason D. Williams, Mercedes Linares-Moreau, chiara parise, Weibin Liang, Heinz Amenitsch, Christian Doonan, C. Oliver Kappe, paolo falcaro
Zeolitic Imidazolate Framework (ZIF) biocomposites show the capacity to protect and deliver bio-therapeutics. To date, the progress in this research area is based on laboratory batch methods. To further explore the potential of ZIF-biocomposites for application to biomedicine and biotechnology, the continuous production of ZIF-biocomposites of specific particle size is desirable. We report the first continuous flow synthetic method for the encapsulation of a model protein (BSA) and a clinical therapeutic (α1-antitrypsin, AAT) in ZIF-8. We studied the in situ kinetics of nucleation, growth and crystallization of BSA-ZIF-8 by SAXS. By controlling the injection time of ethanol, we could quench the particle growth via ethanol-induced crystallization. The particle size of the biocomposite was tuned in the 40-100 nm range by varying residence time prior to introduction of ethanol.


Email Address of Submitting Author


TU Graz



ORCID For Submitting Author


Declaration of Conflict of Interest

no conflicts to declare

Version Notes

Corresponding authors: P. Falcaro and C.O. Kappe