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Continuous Flow Synthesis of ZIF-8 Biocomposites with Tuneable Particle Size

preprint
submitted on 17.01.2020 and posted on 21.01.2020 by Francesco Carraro, Jason D. Williams, Mercedes Linares-Moreau, chiara parise, Weibin Liang, Heinz Amenitsch, Christian Doonan, C. Oliver Kappe, paolo falcaro
Zeolitic Imidazolate Framework (ZIF) biocomposites show the capacity to protect and deliver bio-therapeutics. To date, the progress in this research area is based on laboratory batch methods. To further explore the potential of ZIF-biocomposites for application to biomedicine and biotechnology, the continuous production of ZIF-biocomposites of specific particle size is desirable. We report the first continuous flow synthetic method for the encapsulation of a model protein (BSA) and a clinical therapeutic (α1-antitrypsin, AAT) in ZIF-8. We studied the in situ kinetics of nucleation, growth and crystallization of BSA-ZIF-8 by SAXS. By controlling the injection time of ethanol, we could quench the particle growth via ethanol-induced crystallization. The particle size of the biocomposite was tuned in the 40-100 nm range by varying residence time prior to introduction of ethanol.

History

Email Address of Submitting Author

francesco.carraro@tugraz.at

Institution

TU Graz

Country

Austria

ORCID For Submitting Author

0000-0001-8485-4676

Declaration of Conflict of Interest

no conflicts to declare

Version Notes

Corresponding authors: P. Falcaro and C.O. Kappe

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