ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
1/1
2 files
0/0

Constitutional Isomerization Enables Ultrabright NIR-Ⅱ AIEgen for Brain-Inflammation Imaging

preprint
submitted on 04.07.2019 and posted on 05.07.2019 by shunjie liu, chao chen, yuanyuan li, Haoke Zhang, Junkai Liu, Sherman Wong, Jacky W. Y. Lam, Dan Ding, Ben Zhong Tang
Although biological fluorescence imaging in the second near-infrared window (NIR-Ⅱ, 1000-1700 nm) has embodied the advantages of deep tissue penetration, low background noise and high spatial resolution, shortage of high quantum yield (QY) organic fluorophores has become the bottleneck for further development to this field. Now, an ingenious strategy is proposed to address this: constitutional isomerization by utilizing the molecular design philosophy of aggregation-induced emission (AIE). By solely shifting alkyl chain from meta to ortho position, the resulting twisted structure drives the NIR-Ⅱ fluorophores from aggregation-caused quenching (ACQ) to AIE. In this way, the AIE luminogen (AIEgen), 2TT-oC6B, is provided with an emission peak at 1030 nm and a QY of 11% in nanoparticles, one of the highest reported so far. To endow the targeting ability to deeply located diseases, neutrophils (NEs), the most common immune cells, are used to penetrate the brain tissues. Herein, we show that NEs carrying 2TT-oC6B NPs can penetrate the brain and visualize the deeply located inflammation through intact scalp and skull (3 mm of depth). Notably, the bright 2TT-oC6B fluorophore contributes to a significantly enhanced signal-to-noise ratio of 30.6 in brain inflammation site, which is among the best ones to date. This modern molecule design philosophy could enable a powerful platform for high QY fluorophores and their potential biological applications.

Funding

the National Natural Science Foundation of China

National Key Research and Development program of China

Research Grants Council of Hong Kong

the Innovation and Technology Commission

the National Basic Research Program of China

the Science and Technology Plan of Shenzhen

History

Email Address of Submitting Author

shunjieliu@ust.hk

Institution

the Hong Kong University of Science and Technology

Country

China

ORCID For Submitting Author

0000-0003-1632-0183

Declaration of Conflict of Interest

no conflict of interest

Exports