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Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications.pdf (282.19 kB)
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Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications

preprint
revised on 12.06.2020 and posted on 16.06.2020 by Jonathan Bruce, Jude Clapper

Quantum dots have proven to be strong candidates for biosensing applications in recent years, due to their strong light emission properties and their ability to be modified with a variety of functional groups for the detection of different analytes. Here, we investigate the use of conjugated carboxylated graphene quantum dots (CGQDs) for the detection of E. coli, using a biosensing procedure that focuses on measuring changes in fluorescence quenching. We have also further developed this biosensing assay into a compact, field-deployable test kit focused on rapidly measuring changes in absorbance to determine bacterial concentration. Our CGQDs were conjugated with cecropin P1, a naturally-produced antibacterial peptide that facilitates the attachment of CGQDs to E. coli cells. We also confirm the structural modification of these conjugated CGQDs in addition to analyzing their optical characteristics. Our findings have the potential to be used in situations where rapid, reliable detection of bacteria in liquids, such as drinking water, is required, especially given our biosensor’s relatively low observed limit of detection (LOD).


History

Email Address of Submitting Author

jbruce1461@gmail.com

Institution

Taipei American School

Country

Taiwan

ORCID For Submitting Author

0000-0002-9906-6995

Declaration of Conflict of Interest

The authors have declared that no competing interests exist

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