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Comparative Analysis of Maleic Acid Copolymer-Based Lipid Nanodiscs Reveals Preferential Lipid and Protein Solubilization

preprint
submitted on 19.03.2020 and posted on 19.03.2020 by Marta Barniol-Xicota, Steven Verhelst
Membrane proteins are key in a large number of physiological and pathological processes. Their study often involves a prior detergent solubilization step, which strips away the membrane and can jeopardize membrane protein integrity. A recent alternative to detergents encompasses maleic acid based copolymers (xMAs), which disrupt the lipid bilayer and form lipid protein nanodiscs (xMALPs) soluble in aqueous buffer. Although xMALPs are often referred to as native nanodiscs, little is known about the resemblance of their lipid and protein content to the native bilayer. Here we have analyzed prokaryotic and eukaryotic xMALPs using lipidomics and in-gel analysis. Our results show that the xMALPs content varies with the chemical properties of the used xMA and that some of these nanodiscs are less native than initially thought.

History

Email Address of Submitting Author

steven.verhelst@kuleuven.be

Institution

KU Leuven

Country

Belgium

ORCID For Submitting Author

0000-0002-7400-1319

Declaration of Conflict of Interest

No conflict of interest

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